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產(chǎn)品展示

人甲狀腺刺激素抗體;甲狀腺刺激免疫球蛋白(TSAb)ELISA 檢測(cè)試劑盒

人甲狀腺刺激素抗體;甲狀腺刺激免疫球蛋白(TSAb)ELISA  檢測(cè)試劑盒

價(jià)格:電議 型號(hào):

原產(chǎn)地:中國(guó)大陸發(fā)布時(shí)間:2022/3/24 15:28:39

人甲狀腺刺激素抗體;甲狀腺刺激免疫球蛋白(TSAb)ELISA 檢測(cè)試劑盒;
試劑盒采用雙抗體夾心法酶聯(lián)免疫吸附試驗(yàn)(ELISA)。往預(yù)先包被人甲狀腺刺激素抗體;甲狀腺刺激免疫球蛋白(TSAb)捕獲抗體的 包被微孔中,依次加入標(biāo)本、標(biāo)準(zhǔn)品、HRP標(biāo)記的檢測(cè)抗體,經(jīng)過(guò)溫 育并徹底洗滌。用底物TMB顯色,TMB在過(guò)氧化物酶的催化下轉(zhuǎn)化成 藍(lán)色,并在酸的作用下轉(zhuǎn)化成*終的黃色。

采購(gòu)度:153

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詳細(xì)內(nèi)容

本公司3月24日15時(shí)30分報(bào)道:(http://www.app17.com/c110001/products/d11314676.html)

 
 

2. 實(shí)驗(yàn)中不用的板條應(yīng)立即放回自封袋中,密封低溫干燥保存。

3. 預(yù)處理后的樣本無(wú)需稀釋,直接取 10μL 加樣即可。

4. 嚴(yán)格按照說(shuō)明書(shū)中標(biāo)明的時(shí)間、加液量及順序進(jìn)行溫育操作。

5. 所有液體組分使用前充分搖勻。

 試劑盒組成

 

名稱

96 孔配置

48 孔配置

備注

微孔酶標(biāo)板

12 孔×8 條

12 孔×4 條

無(wú)

標(biāo)準(zhǔn)品

0.3mL

0.3mL

無(wú)

樣本稀釋液

6mL

3mL

無(wú)

檢測(cè)抗體-HRP

10mL

5mL

無(wú)

20×洗滌緩沖液

25mL

15mL

按說(shuō)明書(shū)進(jìn)行稀釋

底物A

6mL

3mL

無(wú)

底物B

6mL

3mL

無(wú)

終止液

6mL

3mL

無(wú)

封板膜

2 張

2 張

無(wú)

說(shuō)明書(shū)

1 份

1 份

無(wú)

自封袋

1 個(gè)

1 個(gè)

無(wú)

注:標(biāo)準(zhǔn)品濃度依次為:20、10、5、2.5、1.25、0 ng/mL.

 試劑的準(zhǔn)備

20×洗滌緩沖液的稀釋:蒸餾水按 1:20 稀釋,即 1 份的 20×洗滌緩沖液加 19 份的蒸餾水。

 洗板方法

1. 手工洗板:甩盡孔內(nèi)液體,每孔加滿洗滌液,靜置 1min 后甩盡孔內(nèi)液體,在吸水紙上拍干,如此洗板 5 次。

2. 自動(dòng)洗板機(jī):每孔注入洗液 350μL,浸泡 1min,洗板 5 次。

 操作步驟

1. 從室溫平衡 20min 后的鋁箔袋中取出所需板條,剩余板條用自封袋密封放回 4℃。

2. 設(shè)置標(biāo)準(zhǔn)品孔和樣本孔,標(biāo)準(zhǔn)品孔各加不同濃度的標(biāo)準(zhǔn)品 50μL;

3. 待測(cè)樣本孔先加待測(cè)樣本 10μL,再加樣本稀釋液 40μL;

4. 隨后標(biāo)準(zhǔn)品孔和樣本孔中每孔加入辣根過(guò)氧化物酶HRP)標(biāo)記的檢測(cè)抗體  100μL,用封板膜封住反應(yīng)孔,37℃水浴鍋或恒溫箱溫 60min。

5. 棄去液體,吸水紙上拍干,每孔加滿洗滌液,靜置 1min,甩去洗滌液,吸水紙上拍干,如此重復(fù)洗板 5 也可用洗板機(jī)洗板

6. 每孔加入底物AB  50μL,37℃避光孵育 15min。


7. 每孔加入終止液 50μL,15min 內(nèi),在 450nm 波長(zhǎng)處測(cè)定各孔的 OD 值。

 結(jié)果判斷


繪制標(biāo)準(zhǔn)曲線:在 Excel 工作表中,以標(biāo)準(zhǔn)品濃度作橫坐標(biāo),對(duì)應(yīng)OD 值作縱坐標(biāo),繪制出標(biāo)準(zhǔn)品線性回歸曲線,按曲線方程計(jì)算各樣本濃度值。

 試劑盒性能

1. 準(zhǔn)確性:標(biāo)準(zhǔn)品線性回歸與預(yù)期濃度相關(guān)系數(shù) R 值,大于等于0.9900。

2. 靈敏度:檢測(cè)濃度小于 0.1 ng/mL。

3. 特異性:不與其它可溶性結(jié)構(gòu)類似物交叉反應(yīng)。

4. 重復(fù)性:板內(nèi)變異系數(shù)小于 10%、板間變異系數(shù)小于 15%。

5. 貯藏:2-8℃,避光防潮保存。

6. 有效期:6 個(gè)月

 免責(zé)聲明

1. 試劑盒僅供研究使用,不得用于臨床實(shí)驗(yàn)或人體實(shí)驗(yàn),否則所產(chǎn)生的一切后果,由實(shí)驗(yàn)者承擔(dān),本公司概不負(fù)責(zé)。

2. 嚴(yán)格按照說(shuō)明書(shū)操作,實(shí)驗(yàn)者違反說(shuō)明書(shū)操作,后果由實(shí)驗(yàn)者承擔(dān)。


FOR RESEARCH USE ONLY.

NOT FOR USE IN DIAGNOSTIC PROCEDURES.

 

Human Thyroid stimulating antibody (TSAb) ELISA Kit instruction

 

Intended use

This TSAb ELISA kit is intended Laboratory for Research use only and is  not for use in diagnostic or therapeutic procedures. The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of TSAb in  the sample, this TSAb ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus TSAb concentration. The concentration of TSAb in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Sample collection and storages

Serum - Use a serum separator tube and allow samples to clot for 30 minutes before centrifugation for 10 minutes at approximately 3000×g. Remove serum and

assay immediately or aliquot and store samples at -20or -80.Avoid repeated freeze-thaw cycles

Plasma - Collect plasma using EDTA or heparin as an anticoagulant. Centrifuge samples for 30 minutes at 3000×g at 2-8within 30 minutes of collection. Store samples at -20or -80. Avoid repeated freeze-thaw cycles.

Cell culture supernates and other biological fluids - Remove particulates by centrifugation and assay immediately or aliquot and store samples at -20or

-80. Avoid repeated freeze-thaw cycles.

    Note:The samples shoule be centrifugated  dequately and  no hemolysis or granule was allowed.

Materials required but not supplied

1. Standard microplate reader(450nm)

 

2. Precision pipettes and Disposable pipette tips.

3. 37  incubator

 

Precautions

1. Do not substitute reagents from one kit to another. Standard, conjugate and microplates are matched for optimal performance. Use only the reagents supplied by manufacturer.

2. Do not remove microplate from the storage bag until needed. Unused strips


should be stored at 2-8°C in their pouch with the desiccant provided.

 

3. Mix all reagents before using.

 

Remove all kit reagents from refrigerator and allow them to reach room temperature  ( 20-25°C)

Materials supplied

 

Name

96 determinations

48 determinations

Microelisa stripplate

12*8strips

12*4strips

Standard

0.3ml

0.3ml

Sample diluent

6.0ml

3.0ml

HRP-Conjugate reagent

10.0ml

5.0ml

20X Wash solution

25ml

15ml

Chromogen Solution A

6.0ml

3.0ml

Chromogen Solution B

6.0ml

3.0ml

Stop Solution

6.0ml

3.0ml

Closure plate membrane

2

2

User manual

1

1

Sealed bags

1

1

Note: Standard concentration was followed by: 2010、5、2.5、1.25、0 ng/mL.

Reagent preparation

20×wash solution:Dilute with Distilled or deionized water 1:20.

Assay procedure

1. Prepare all r eagen t s before starting assay procedure. It is recommended that all Standards and Samples be added in duplicate to the Microelisa Stripplate.

2. Add standard: Set Standard wells, testing sample wells. Add standard 50μl to standard well.

3. Add Sample: Add testing sample 10μl Then add sample diluent 40μl to testing sample well; Blank well doesn’t add anyting.

4. Add 100μl of HRP-conjugate reagent to each well, cover with an adhesive strip and incubate for 60 minutes at 37°C.

5. Aspirate each well and wash, repeating the process four times for a total of five washes. Wash by filling each well with Wash Solution (400μl) using a squirt bottle, manifold dispenser or autowasher. Complete removal of liquid at each step  is essential to good performance. After the last wash, remove any remaining Wash Solution by aspirating or decanting. Invert the plate and blot it against clean paper towels.

6. Add chromogen solution A 50μl and chromogen solution B 50μl to each well.

 

Gently mix and incubate for 15 minutes at 37°C. Protect from light.

 

7. Add 50μl Stop Solution to each well. The color in the  wells  should  change  from blue to yellow. If the color in the wells is green or the color change does not appear uniform, gently tap the plate to ensure thorough mixing.

 本試劑盒只能用于科學(xué)研究,不得用于醫(yī)學(xué)診斷

更新時(shí)間:2024/5/24 11:45:35

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